Production of non-glycosylated recombinant Pla l 1, the main allergen of Plantago lanceolata
نویسندگان
چکیده
Methods Recombinant Pla l 1.0101 was heterologously expressed in the E. coli strain Rosetta-gami B pLysS and purified using cation exchange and size exclusion chromatography. Natural Pla l 1 was obtained by pollen extraction and cation exchange chromatography. Physico-chemical properties of the purified proteins were analyzed in gel electrophoresis, mass spectrometry and circular dichroism. Using sera from Austrian ribwort pollen allergic patients (n=20) the IgE-binding activity of natural and recombinant Pla l 1 was investigated in ELISA.
منابع مشابه
Molecular aerobiology - Plantago allergen Pla l 1 in the atmosphere.
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